SpecificBacillus anthracisidentification by aplcR-targeted restriction site insertion-PCR (RSI-PCR) assay
نویسندگان
چکیده
منابع مشابه
Restriction site-free insertion of PCR products directionally into vectors.
A restriction site-free cloning method has been developed for inserting a PCR product into a vector flexibly and precisely at any desired location with high efficiency. The method uses a pair of DNA integration primers with two portions. The 3' portion isolates the inserts by PCR, and the 5' portion integrates the PCR products into the homologous region of the vector. For mutagenesis, a third p...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2007
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2007.00741.x